Composite

Part:BBa_K2926029:Design

Designed by: Astrid Többer   Group: iGEM19_Bielefeld-CeBiTec   (2019-10-15)


f1_ori_mCherry-M13K07-pVIII-fusion_pIII


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

lglshgl


Source

The f1 origin of replication and mCherry are from iGEM, the genes VIII and III are fron the M13K07 helperphage from NEB.

References